COM.on C.A.2:e2/11-16 Online published on Jul. 6, 2008.
doi:10.4236/coca.2008.21002
REPORT
 Evidence from Autosomal STR Data Supports a Central China Origin of Chao-Shan Population

XU Lina 1, FENG Guoying1, ZHOU Guangbiao1, HU Shengping1, 2

1.Center for Molecular Biology, 2.Forensic Genetics Laboratory, Shantou University Medical College, Shantou, Guangdong 515031 China

　

ABSTRACT: Chao-Shan people are typical Southern Han Chinese, and numbers of them emigrate to many other countries. The origin of Chao-Shan people is believed to be in Central China. The comparative study of the genetic polymorphism of Henan and Chao-Shan populations can test the hypothesis of Central China origin of Chao-Shan people at the genomic level. Here we performed a combined method of fluorescence labeled PCR and capillary electrophoresis to study the genetic polymorphism of 15 STR loci in 200 unrelated healthy individuals in Henan Han population. Combining our published STR allele frequency data and the new data from additional samples in Chao-Shan population and STR data of other populations in different areas obtained from peer-reviewed journals, we calculated the Nei’s genetic distance D’ between the populations with the PHYLIP software. The neighbor-joining phylogenetic tree was constructed based on the obtained Nei’s genetic distance to reveal population affinities and the Central China origin of Chao-Shan people. A total of 142 alleles and 451 genotypes were detected in Henan population. No deviation from Hardy-Weinberg equilibrium was observed in STR loci except for D3S1358. The phylogenic tree based on CODIS STR allele frequencies showed that Chao-Shan Han was much closer to Minnan Han, and relatively closer to Henan Han. Admixture analysis indicated that Chao-Shan Han and Minnan Han have similar proportional contributions from the Central China Han and the proportion was greater than 60%. Distinction between north and south populations in China was noticeable in the phylogenic tree. The 15 STR loci studied have potential usefulness for parental testing and forensic personal identification in Henan population. Our study showed the genetic similarity sustained by Chao-Shan Han and Minnan Han, and a pronounced Central China Han ancestry on the gene pools of the two groups.

Key words: Population genetics; Short tandem repeat (STR); Chao-Shan population; Henan population; Polymerase chain reaction (PCR)

　

Recieved: May 23, 2008 Accepted: Jun. 30,2008 Corresponding: sphu@stu.edu.cn

《现代人类学通讯》第二卷e2篇 第11-16页 2008年7月6日网上发行

研究报告

常染色体STR的证据支持潮汕人的中原汉族起源

许丽娜1，冯国英1，周广彪1，2，胡盛平1，2

1 汕头大学医学院分子生物学中心，广东汕头 515031；2.汕头大学司法鉴定中心DNA 物证研究室，广东汕头 515031

　

摘要: 潮汕人群是典型的中国南方汉族群体，并且大规模移居海外。一般认为潮汕人群起源于河南一带。在DNA水平上比较潮汕和河南人群的遗传多态性，可以深入探究潮汕人的中原汉族起源。我们采用荧光标记PCR和毛细管电泳相结合的技术对河南汉族200例健康人群的15个STR基因座遗传多态性进行研究，结合本课题组已发表潮汕人群STR数据并新增样本以及搜集到的发表在国内外刊物的其它地区人群STR数据，用Phylip软件计算不同人群间的遗传距离，构建高分辨率进化树,探讨潮汕人群的中原汉族起源及与其他人群之间的关系。河南汉族人群15个STR基因座共检出142个等位基因451种基因型，其观察值与期望值经精确检验，均无显著性差异（D3S1358除外）。选取CODIS系统STR基因座的人群数据聚类分析结果显示潮汕汉族离闽南汉族很近，距河南汉族较近；融合分析显示潮汕和闽南这两个群体具有相似的中原汉族成分且中原汉族的成分所占比例均大于60％。聚类图上中国人群的南北分界明显。河南汉族人群15个STR基因座联合应用可满足河南人群法医学的个体识别及亲子鉴定的需要；潮汕和闽南这两个群体具有一贯的遗传相似性，且占有较大的中原汉族起源成分。

关键词: 群体遗传学；短串联重复序列；潮汕人群；河南人群；聚合酶链式反应

　

收稿日期：2008年5月23日 修回日期：2008年6月30日 联系人:胡盛平 sphu@stu.edu.cn
作者简介：许丽娜(1982-)，女，群体遗传学硕士；Tel：0754-8900406; E-mail: lnxu2005@163.com

全文链接 Full text: [PDF]
　

基金支持: 香港李嘉诚基金会剑桥大学国际合作项目基金；英国剑桥大学基金；广东省自然科学基金（000819）；汕头大学研究与发展基金（L00007）；汕头市重点科技计划项目(汕府科[2005]116 号)。

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