COM.on C.A.3:e12/100-109     Online published on Nov.18, 2009.
doi:10.4236/coca.2009.31012
REPORT
 Origins of the Workers Who Built the First Qin Emperor's Mausoleum Revealed by Mitochondrial DNA

YUAN Yuan1, XU Zhi1, TAN Jingze1, ZHANG Fan1, XU Bosong1, LI Shilin1, LI Chunxiang2, ZHOU Hui2, ZHU Hong2, ZHANG Jun3, DUAN Qingbo4, JIN Li1,5, LI Hui1

1. MOE Key Laboratory of Contemporary Anthropology and Center for Evolutionary Biology, School of Life Sciences and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433 China; 2. Ancient DNA Laboratory, Research Center for Chinese Frontier Archaeology, Jilin University, Changchun JL130012 China; 3. Institute of Archaeology, Chinese Academy of Social Sciences, Beijing 100710 China; 4. Shaanxi Provincial Institute of Cultural Relics and Archaeology, Xi’an SA710054 China; 5. Chinese Academy of Sciences and Max Planck Society Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 China.

ABSTRACT: Ancient DNA analyses are always employed to reveal the ethnic origins of the ancient human remains. In this paper, we analyzed 19 human bone remains excavated from a tomb near the Qin Dynasty’s Terra-Cotta Warriors and dated some 2,200 years before present. There samples were supposed to be workers building the mausoleum for the First Emperor of China. The polymorphisms in the first hypervariable region and coding regions of mitochondrial DNA were analyzed using cloning sequencing and RFLP. The results were compared with 2,164 mtDNA profiles from 32 contemporary Chinese populations at both population and individual levels. Our results showed that mausoleum-building workers may be originated from very diverse ethnic populations.

Key words: Ancient DNA; mtDNA; Mausoleum-building workers (MBWs); the First Emperor of China

Recieved: Nov.11, 2009   Accepted: Nov. 16,2009  Corresponding: tanjingze@gmail.com

《现代人类学通讯》第三卷e12篇 第100-109页  2009年11月18日网上发行

研究报告

线粒体证据揭示秦陵修陵劳工的来源

袁媛1, 徐智1, 谭婧泽1, 张帆1, 许渤松1, 李士林1, 李春香2, 周惠2, 朱泓2, 张君3, 段清波4, 金力1,5, 李辉1

1.复旦大学 生命科学学院 生物医学研究院 现代人类学教育部重点实验室 进化生物学中心，上海 200433；2.吉林大学 边疆考古研究中心 古DNA实验室，长春 130012；3.中国社会科学院 考古研究所，北京 100710；4.陕西省文物与考古研究所，西安 710054；5.中国科学院 上海生命科学研究院-马普学会 计算生物学伙伴研究所，上海 200031.

摘要：越来越多的研究试图利用古DNA的手段揭示古代人类个体的来源。2003年秦始皇陵秦俑坑附近出土的一批人骨被推测是建陵劳工的遗骸，年代大约在2200年前。我们通过克隆测序和PCR-PRLP的方法，成功地从19个骨样本中得到线粒体HVR区和编码区的SNP位点的序列信息。为了揭示这些劳工的来源，我们将所得序列与中国现代人的32个群体(2164个体)的线粒体序列分别在个体和群体水平上作比较，结果表明秦陵劳工墓的劳工来源是多样的。本文详尽地介绍了古DNA的操作方法、序列信息的甄别和数据分析方法。

关键词：古DNA；线粒体DNA；秦始皇陵劳工

收稿日期：2009年11月11日  修回日期：2009年11月16日 联系人：谭婧泽 tanjingze@gmail.com

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致谢：本研究得到国家自然科学基金(30571013)，上海市浦江人才计划(05pj14023)，国家杰出青年基金(30625016)的资助。

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