WANG Chuanchao,LI Hui
MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai 200433, China.
Key words: Ancient DNA, Molecular cloning, Polymerase chain reaction (PCR), Next generation DNA sequencing techniques, Primer extension capture (PEC), Array hybridization capture(AHC).
Recieved: May.10, 2010 Accepted: May.25, 2010 Corresponding: lihui.fudan@gmail.com
《现代人类学通讯》第四卷e6篇 第34-41页 2010年6月2日网上发行
专题综述
古DNA分析技术发展的三次革命
王传超, 李辉
复旦大学现代人类学教育部重点实验室,上海200433.
摘要:古DNA研究以分子生物学技术为基础,以古生物的DNA为研究对象,是一个新兴领域。20余年来,古DNA实验技术不断发展。分子克隆、PCR、下一代测序技术、引物延伸捕获和芯片杂交捕获等扩增和测序技术的不断涌现,分别引领了古DNA研究的三次革命,极大推动古DNA研究发展、成熟。从229bp斑驴的mtDNA到尼安德特人基因组草图,古DNA研究取得一系列的突出成果, 解开了众多千万年来的谜题。
关键词:古DNA;分子克隆;聚合酶链式反应(PCR);下一代测序技术;引物延伸捕获(PEC);芯片杂交捕获(AHC)
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