YUAN Yuan
MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai 200433 China
Key words: ancient DNA; contamination; high-throughput sequencing
Recieved: Jun.28, 2010 Accepted: Jul.14, 2010 Corresponding: yuanyuan.fudan@gmail.com
《现代人类学通讯》第四卷e15篇 第96-100页 2010年7月16日网上发行
专题综述
控制古DNA污染的方法
袁媛
复旦大学 生命科学学院 现代人类学教育部重点实验室,上海,200433
摘要:控制污染是古DNA研究的难题之一。当前,高通量测序提高了古DNA检测灵敏度的同时使污染分子大量扩增,这使控制和检测古DNA污染更加重要。先前的古DNA行业标准基于PCR反应,所以对现行的古DNA研究仍适用。现在针对高通量测序,古DNA研究又发展了新的减少和检测污染的手段。目前,证明古DNA可靠性的方法,主要有检测片段长度、DNA的错配模式、片段末端5'和3'的碱基频率、测序覆盖率与GC含量的相关性。而对于样本的检测前污染,染色体的杂合态分析可以一定程度上判断污染状况。在现阶段的研究条件下,多方面的证据的支持更有利于判定所得的古DNA序列是否可靠。
关键词:古DNA;污染;高通量测序
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